表皮生长因子途径底物蛋白1(EPN1)重组蛋白

YB141Hu013
Recombinant Epsin 1 (EPN1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 表皮生长因子途径底物蛋白1(EPN1)重组蛋白[ PROPERTIES ]
Residues: Pro279~Gly437 (Accession # Q9Y6I3),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasm. Cell
membrane; Peripheral membrane protein.
Nucleus. Membrane, clathrin-coated pit.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 表皮生长因子途径底物蛋白1(EPN1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 4.3
Predicted Molecular Mass: 16.9kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
 表皮生长因子途径底物蛋白1(EPN1)重组蛋白protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- PA PMAAAVPTAA PTSDPWGGPP VPPAADPWGG PAPTPASGDP
WRPAAPAGPS VDPWGGTPAP AAGEGPTPDP WGSSDGGVPV SGPSAS DPWT
PAPAFSDPWG GSPAKPSTNG TTAAGGFDTE PDEFSDFDRL RTALPTSGSS AGELELLAGE
VPARSPG