整合素αD(ITGαD)重组蛋白

YB271Ra011
Integrin Alpha D (ITGaD)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Phe879~Asn1099 (Accession # Q13349),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 29.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
整合素αD(ITGαD)重组蛋白(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
整合素αD(ITGαD)重组蛋白Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
整合素αD(ITGαD)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGS S H HH HH H SSG LVP R GS H MA SM TG G Q QM GR GSEF - FD VSY KAT LG D R
MLMRASASSE NNKASSSKAT FQLELPVKYA VYTMISRQEE STKYFNFATS DEKKMKEAEH
RYRVNNLSQR DLAISINFWV PVLLNGVAVW DVVMEAPSQS LPCVSERKPP QHSDFLTQIS
RSPMLDCSIA DCLQFRCDVP SFSVQEELDF TLKGNLSFGW VRETLQKKVL VVSVAEITFD
TSVYSQLPGQ EAFMRAQMEM VLEEDEVYN