11-β-羟基类固醇脱氢酶1(HSD11β1)重组蛋白

YB268Ra011
Recombinant 11-Beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11b1)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 11-β-羟基类固醇脱氢酶1(HSD11β1)重组蛋白[ PROPERTIES ]
Residues: Met1~Asn288
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P16232
Host: E. coli
Subcellular Location: Endoplasmic reticulum
membrane; Single-pass type II membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
 11-β-羟基类固醇脱氢酶1(HSD11β1)重组蛋白Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.6
Predicted Molecular Mass: 35.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
 11-β-羟基类固醇脱氢酶1(HSD11β1)重组蛋白The sequence of the target protein is listed below.
MKKYLLPVLV LCLGYYYSTN EEFRPEMLQG KKVIVTGASK GIGREMAYHL SKMGAHVVLT
ARSEEGLQKV VSRCLELGAA SAHYIAGTME DMAFAERFVV EAGKLLGGLD MLILNHITQT
TMSLFHDDIH SVRRSMEVNF LSYVVLSTAA LPMLKQSNGS IAIISSMAGK MTQPLIASYS
ASKFALDGFF STIRKEHLMT KVNVSITLCV LGFIDTETAL KETSGIILSQ AAPKEECALE
IIKGTVLRKD EVYYDKSSWT PLLLGNPGRR IMEFLSLRSY NRDLFVSN