PPEF1 Validated Chimera RNAi
产品名称： PPEF1 Validated Chimera RNAi
英文名称： PPEF1 Validated Chimera RNAi
产品编号： H00005475-R01V
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Homo sapiens protein phosphatase, EF-hand calcium binding domain 1 (PPEF1), transcript variant 2, mRNA.
- Reactivity:
- Human
- Storage Instruction:
- Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure product integrity.
- Supplied Product:
- DEPC water
- Target Refseq:
- NM_006240
- Note:
- Position of the Chimera RNAi.
-
- Publication Reference
- 1.
- dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.
- 2.
- Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect.
Ui-Tei K, Naito Y, Zenno S, Nishi K, Yamato K, Takahashi F, Juni A, Saigo K.Nucleic Acids Res. 2008 Apr;36(7):2136-51. Epub 2008 Feb 11.
- 3.
- Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004.
- 4.
- siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference.
Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.
- Applications
- RNAi Knockdown (Antibody validated)
- Western blot analysis of PPEF1 over-expressed 293 cell line, cotransfected with PPEF1 Validated Chimera RNAi ( Cat # H00005475-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPEF1 monoclonal antibody (M01), clone 1F6-1A5 (Cat # H00005475-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 5475
- Gene Name:
- PPEF1
- Gene Alias:
- PP7,PPEF,PPP7C
- Gene Description:
- protein phosphatase, EF-hand calcium binding domain 1
- Omim ID:
- 300109
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq
- Other Designations:
- OTTHUMP00000023006,protein phosphatase with EF hand calcium-binding domain 1,protein phosphatase, EF hand calcium-binding domain 1,protein phosphatase, serine/threonine type, with EF-hands,serine/threonine protein phosphatase 7