Description

Renal mesangial cells are perivascular cells located within the central portion of the glomerular tuft between capillary loops, constituting 30-40% of the total glomerular cell population [1]. Mesangial cells synthesize, assemble and control turnover of the mesangial matrix [2]. They also regulate the intraglomerular capillary flow and ultrafiltration surface via mesangial cell contraction and release of various growth factors and vasoactive agents [3, 4]. By pinocytosis and phagocytosis, mesangial cells remove local accumulation of macromolecules in the mesangial space [5, 6]. Dysregulation of such housekeeping functions results in release of proinflammatory mediators and leukocyte infiltration, subsequently affects glomerular permselectivity to cause proteinuria. Furthermore, mesangial cell proliferation and matrix overproduction have been observed in a wide variety of glomerular diseases, such as IgA nephropathy, mesangioproliferative glomerulonephritis, lupus nephritis, glomerulosclerosis and diabetic nephropathy [7, 8]. Such critical involvements suggest renal mesangial cells as an ideal model for studying mesangial injury and glomerular functions under both physiological and pathophysiological conditions.

RRMC from ScienCell Research Laboratories are isolated from neonatal day 2 rat renal tissue. RRMC are cryopreserved after purification and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. RRMC are characterized by immunofluorescence with antibodies specific to fibronectin, Thy-1, and smooth muscle actin. RRMC are negative for mycoplasma, bacteria, yeast and fungi. RRMC are guaranteed to further expand for 5 population doublings in the condition provided by ScienCell Research Laboratories.

Recommended Medium

It is recommended to use Mesangial Cell Medium (MCM, Cat. No. 4201) for the culturing of RRMC in vitro.

Product Use

RRMC are for research use only. They are not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Transfer cells directly and immediately from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.

Shipping

Dry ice.

Reference

[1] Olivetti G, Anversa P, Rigamonti W, et al. (1977) J Cell Biol. 75(2 Pt 1):573-85.
[2] Couchman JR, Beavan LA and McCarthy KJ. (1994) Kidney Int. 45:328-35.
[3] Riser BL, Cortes P, Heilig C, et al. (1996) Am J Pathol. 148:1915-23.
[4] Gruden G, Thomas S, Burt D, et al. (1999) J Am Soc Nephrol 10:730-37.
[5] Wanner C, Greiber S, Kramer-Guth A, et al. (1997) Kidney Int Suppl 63:S102-6.
[6] Gomez-Guerrero C, Suzuki Y and Egido J. (2002) Kidney Int 62:715-7.
[7] Schlöndorff D and Banas B. (2009) J Am Soc Nephrol. 20:1179-87.
[8] Abboud HE. (2012) Exp Cell Res. 318:979-85.