Bcl2关联X蛋白(Bax)重组蛋白

YB343Ra011
Recombinant Bcl2 Associated X Protein (Bax)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 Bcl2关联X蛋白(Bax)重组蛋白[ PROPERTIES ]
Residues: Met1~Gly192 (Accession # Q07814),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Mitochondrion membrane;
Single-pass membrane protein. Cytoplasm. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.9
 Bcl2关联X蛋白(Bax)重组蛋白Predicted Molecular Mass: 22.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 Bcl2关联X蛋白(Bax)重组蛋白[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF- MDGSGEQPRG GGPTSSEQIM KTGALLLQGF IQDRAGRMGG
EAPELALDPV PQDASTKKLS ECLKRIGDEL DSNMELQRMI AAVDTDSPRE VFFRVAADMF
SDGNFNWGRV VALFYFASKL VLKALCTKVP ELIRTIMGWT LDFLRERLLG WIQDQGGWDG
LLSYFGTPTW QTVTIFVAGV LTASLTIWKK MG